Anti-angiogenic activity of Ficus deltoidea L. Jack silver nanoparticles using chorioallantoic membrane assay

Background: Ficus deltoidea L. Jack is a folk medicinal plant known for its pharmacological properties, including anti-inflammatory, anticancer and anti-angiogenic. This study aimed to evaluate the anti-angiogenic properties of silver nanoparticles biosynthesized using F. deltoidea leaf extract (AgNPs-Fd). Methods: The AgNPs-Fd were synthesized by mixing 100 mL 1 mM aqueous nitrate (AgNO3) 0.1% ethanolic extract. The resulting observed color change Tyndall effects. Reaction mixture from pale brown reddish was at 48 h 37°C. characterization completed with UV–Vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) Fourier transform infrared (FTIR) spectroscopy. For quantitative analysis vascular network in chorioallantoic membrane (CAM) assay, AngioTool open-source software used. Results: plasmon resonance peak 430 nm visible UV-Visible spectrum, indicating formation AgNPs-Fd. interacted well according FTIR analysis. morphology 20 particle sizes TEM. chromatographic identified potential compounds, such as phytol, stigmasterol, lupeol sitosterol. angiogenic inhibition tested CAM assay. 90 mg dose treatment CAMs demonstrated significant anti-angiogenesis, effectiveness controlling vessel formation. Conclusions: present suggests that eco-friendly work “green” process potentially applicable nanobiotechnology fields.